Stevia (Stevia rebaudiana) is a herbaceousplant species which belongs to
Asteraceae family which has medicinal properties. This plant has a sweet taste
inits leaves which contain steviol glycosides. The sweetener in stevia leaves is a
non-calorie natural sweetener so it is safe for people with type 2 diabetes mellitus.
The sweetener may also reduce obesity. Stevia propagation by seed faces low
germination rate of seeds and having high variety of its seedlings. Propagation by
cuttings is limited by the number of plant sources. Another method for mass
propagation is by tissue culture techniques. In stevia, tissue culture has been
applied since the 1970s and up to now is still being developed with various
modifications such asby altering media compositions, the growing environment,
and the cultivation system. Propagation of stevia using a liquid culture system is
one method for mass propagation in the laboratory. Research on liquid culture
has been widely reported. Several researchers have published the use of
bioreactors to increase the production of biomass and plant chemicals including
for stevia. This paper consist of a Literature Review on the micropropagation of
stevia in liquid media. A result showed that the optimal medium for stevia shoot
induction was MS medium containing 1 mg/L BAP or with addition of 1 mg/L
BAP in combination with 0,5 mg/L IAA using apical shoots as explants. Shoots
and roots of Stevia developed simultaneously in a bioreactor containing 100 mL
WPM at a soaking frequency of 10 seconds/4 hours.The healthiest plantlets were
obtained in the bioreactor containing 5 mL WPM medium per explant.