Yuda, Ign. Pramana and Kinanti, Regine Archadia and Saputra, Andie Wijaya (2019) Use of swab for DNA sampling from confiscated raptors for molecular sexing. IOP Conference Series: Earth and Environmental Science. ISSN 1755-1315

Abstract

The objective of the study was to evaluate the feasibility and efficiency of using swab
to collect tracheal and cloacal epithelial cells of confiscated raptor bird for genetic studies.
Commercial swab kits were used to collect samples from 34 individuals of 10 raptor species, and
as comparison blood samples from the same individual were also analyzed. FavorPrep
Blood
Genomic DNA Extraction Mini Kit dan FavorPrep
TM
Tissue Genomic DNA Extraction Mini Kit
were used respectively to extract DNA from blood and epithelial cells. All DNA extracted from
blood were successfully amplified for assignment of sex. On the other hand, the DNA extracted
from buccal and cloacal swabs were only respectively 71% and 9% successfully amplified. This
result suggests the potential used of buccal swabs for genetics studies of raptor, with further
optimization for a better result.

Item Type:	Article
Subjects:	Teknobiologi > Tekno Lingkungan
Divisions:	Fakultas Teknobiologi > Biologi
Depositing User:	Editor UAJY
Date Deposited:	03 Feb 2021 09:56
Last Modified:	03 Feb 2021 09:56
URI:	https://repository.uajy.ac.id/id/eprint/23141

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