Kaffir lime (Citrus hystrix D. C.) is a citrus plant that is generally used as a flavor
enhancer in food and beverages. This plant is native to Indonesia, which contains
flavonoids, alkaloids, saponins, steroids and tannins. This study aims to determine the
ability of antibacterial extract lime leaves against Pseudomonas aeruginosa and
Staphylococcus epidermidis. Theresearch design was completely randomized
treatment with various concentrations of methanol extract lime leaves. The research
begins with drying leaves the oven at a temperature of 45 ˚C, pulverizing the mesh
61, and extraction by maceration for 48 hours with methanol. This study uses a
completely randomized design with treatments in variation concentrations of 12,5,
25, and 50% concentration were then used to test the antibacterial activity. Inhibition
zone formed from extracts with concentrations of 12,5, 25, and 50% against
Pseudomonas aeruginosa respectively were 0,605, 1,132, and 1,934 cm2, whereas
against Staphylococcus epidermidis produced successive inhibition zone were 0.518,
0.837, and 1.251 cm2. Based on the research that has been done a concentration of
50% was the concentration of the most effective in inhibiting the growth of
Staphylococcus epidermidis and Pseudomonas aeruginosa. Then the smallest
concentration of inhibitory zone, was 12,5% followed by the MIC test. Based on the
results obtained by the MIC value for both types of bacteria is was 7,5%.