Namnam or kopi anjing is the name of a type of fruit trees from family of
legumes (Leguminosae or Fabaceae). This plant is one of the native species in
Indonesia that cointains alkaloid, flavonoid, saponin, steroid and tannin. The purpose
of this study was to determine the ability of antibacterial activity of Namnam leaf
extract against Staphylococcus epidermidis and Pseudomonas aeruginosa. This study
uses a completely randomized design with treatments in variation concentrations of
ethyl acetate extract of namnam leaves. This study begins with the extraction process
with maceration for 5 days using solvent ethyl acetate. Extract devised into various
concentrations of 20, 40, 60 and 80%, and tested for antibacterial activity by the
paper disk method. Size inhibition zone formed from the extract with a concentration
of 20, 40, 60 and 80 % for Staphylococcus epidermidis sequentially was 0.84, 0.94, 1
and 1.3 cm2 while for Pseudomonas aeruginosa sequentially is 0.58, 0.84, 0.98 and
1.16 cm2. Based on the research, 80 % concentration is the concentration of the most
effective in inhibiting Staphylococcus epidermidis and Pseudomonas aeruginosa.
After knows the effective concentration of the extract, continued with testing of
Minimum Inhibitory Concentration (MIC) against both bacteria test with liquid
dilution method and Total Plate Count (TPC). Based on research, MIC for
Staphylococcus epidermidis and Pseudomonas aeruginosa was 80%