Hyperuricemia may result from excessive production of uric acid or a reduced
discharge caused by genetic and metabolic disorders. Uric acid levels can be
lowered by taking herbs such as sambiloto and brotowali which contain alkaloids,
tannins, saponins and flavonoids that are processed by the method of decoction.
This study aims to determine the effectiveness of the decoction of sambiloto and
brotowali when combined directly to lower uric acid in mice and the time required
for sambiloto and brotowali to show the effect of decreased uric acid when
induced into the body of male mice. Induction is done by administering potassium
oksonat to increase uric acid levels. The experimental design used in this study is
completely randomized design, with three variety of combination treatment
decocta and two controls. To reduce levels of uric acid is used in combination
sambiloto and brotowali decocta made with three ratio of 1: 3, 2: 2, and 3: 1 and
the provision of distilled water control and the generic drug allupurinol.
Measurement of uric acid levels using a photometer MICROLAB 300 with
absorbance of 546 nm. Results were analyzed by ANOVA with 95% confidence
level using SPSS version 15.0. It was found that the combination of sambiloto :
brotowali effectively lowered uric acid levels with the highest ratio in reducing
the sambiloto : brotowali 3: 1 as many as 13.16 mg/dl within 1 week.